Composite

Part:BBa_K3105683

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-12)


Expression construct Glox

Glyoxal oxidase (Glox) cloned in-frame with α-factor from Saccharomyces cerevisiae. This construct is controlled by the AOX1 promotor, which is inducible by methanol.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1825
    Illegal BamHI site found at 2529
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3014
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None